University of Heidelberg, is offering:
PhD thesis in "Mechanism and Regulation of Cell Division"
Our group is interested in mitotic spindle assembly and function. In particular, we are trying to elucidate how microtubule behaviour in the mitotic spindle is regulated at the start of chromosome segregation and coordinated with the cell cycle machinery. We recently discovered that spindle microtubule dynamics change independently of
the activation of the central activator of anaphase, the Anaphase Promoting Complex (Reber et al., JCB, 2008). Instead, they are governed by the activation of Calcium-dependent signalling effectors, such as Calcium-Calmodulin- dependent kinase. The challenging next question is now to find out, which microtubule regulators are affected by Calcium-dependent signalling.
We are using a combination of biochemical assays in cell free extracts and an siRNA-based approach in living human cells (Tegha-Dunghu et al., 2008) to identify activities involved in spindle microtubule regulation in anaphase and to understand their regulation at anaphase onset.
Our laboratory is member of the recently founded DKFZ-ZMBH Alliance (http://www.dkfz-zmbh-allianz.de/index_en.html) and is looking forward to receive additional support from the award of a Start-Professorship by the Excellence Initiative of German
Universities. The position for the described project is funded by the DFG according to TVL E13/2 as a basic salary. However, the salary can be increased depending on the qualification of the applicant and the performance during the thesis work.
The ZMBH is equipped with all tools for cutting edge cell biology, including a state-of the art microscopy facility and excellent mass spectrometry unit. PhD students of the ZMBH are integrated into the HBIGS graduate school at the University of Heidelberg. Students in our group get excellent supervision in all technical and conceptual questions. Check out our homepage (http://www.zmbh.uni-heidelberg.de/gruss/default.shtml) to find out more.
We are looking for highly motivated individuals, who are seeking for challenging project in basic science. Insight into protein biochemistry is required, and experience with imaging techniques is an advantage. The candidate should have a MSC in Biology, Molecular Biology, Biochemistry or Biophysics.
Please apply directly to HBIGS (http://www.hbigs.uni-heidelberg.de/) or send informal inquiries to o.gruss@zmbh.uni-heidelberg.de
Methoden:
Monitoring cell division after siRNA knock-down of human gene products, Live-cell imaging in human cells using microtubule marker proteins, Reconstitution of cell division processes in cell free extracts, Protein purification and protein activity assay development.
Anfangsdatum: 1. Maerz 2010
geschaetzte Dauer: 3 years
Bezahlung: TVL E13/2 +
Veroeffentlichungen :
Reber, S., S. Over, I. Kronja and O.J. Gruss (2008). CaM kinase II Initiates meiotic spindle depolymerization independently of APC/C activation. J Cell Biol, 183:1007-17
Tegha-Dunghu, J., B. Neumann, S. Reber, R. Krause, H. Erfle, T. Walter, M. Held, R. Rogers, K. Hupfeld, T. Ruppert, J. Ellenberg and O.J. Gruss (2008). Eml3 is a nuclear microtubule binding protein required for proper alignment of chromosomes in metaphase. J Cell Sci. 121:1718-26.
Homepage: http://www.zmbh.uni-heidelberg.de/gruss/default.shtml
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