Title: Study of SERCA1 and of other Ca2+ ATPases: expression, purification and characterization.
Abstract:
The objectives of this project are:
- to express and purify various Ca2+-ATPases by using the procedure developped in our laboratory in an heterologous system in yeast (Jidenko et al., PNAS, 2005) allowing the obtention of crystals able to diffract X-rays. Their structural and functional characterization will be performed. During the thesis the a main effort will be focused on human SERCA3 . Mutation of this gene have been found in patients suffering from type II diabetis. The work will be i) to clone this isoform in view of heterologous expression in yeast and trials of expression. ii) scale up the procedure to obtain large amount of protein. iii)characterizatio n (iv) crystalization trials
Location:
Institut de Biologie et de Technologies de Saclay
Service de Bioénergétique, Biologie Stucturale, et Mécanismes
Laboratoire de Protéine Membranaires / ATPase Calcium
Centre : Saclay
Contact person:
Marc LE MAIRE
CNRS/Université / DSV/IBITEC-S
CEA/Saclay
Email : marc.lemaire@cea.fr
Phone: 01 69 08 62 43
University/Graduate School: Paris XI
Thesis supervisor:
Marc LE MAIRE
CNRS/Université / DSV/IBITEC-S
CEA/Saclay
Note: Application deadline to the PhD scholarship in Biochemistry, Saclay, France is March end, 2011.
Please quote 10 Academic Resources Daily in your application to this opportunity!
